Application of hospital WeChat official account in health communication under public health emergencies / 中华医学教育探索杂志

During the COVID-19 outbreak, the hospital mainly used the WeChat official account for health communication, and responded to public health demands in public health emergencies in a timely manner. Taking Third-Level First-Class Hospital in Chongqing as the breakthrough point, this paper has discussed the advantages of hospital WeChat official account for health communication during the COVID-19 epidemic, found its insufficiency, and proposed optimized paths in production of high quality content, interaction with users, establishment of WeChat matrix, change of communication style and master of the communication timing, etc. Meanwhile, this paper holds that the communication practice of hospital WeChat official account based on public health emergencies has important enlightenment for medical education.

Non-invasive prenatal diagnosis for beta-thalassemia by detecting paternal CD41-42 mutation in cell-free DNA derived from maternal plasma with droplet digital PCR / 中华医学遗传学杂志

OBJECTIVE@#To establish a non-invasive method for beta-thalassemia by detecting parental CD41-42 mutation in cell-free DNA derived from maternal plasma with droplet digital PCR (ddPCR).@*METHODS@#Beta-actin gene and beta-thalassemia gene CD41-42 mutation were respectively set as the reference and target sequences. A novel method was established based on Bio-Rad ddPCR technique with specific primers and TaqMan probes for the two genes. The accuracy, sensitivity and detective linearity range of the developed method were evaluated by detection of the target gene gradient concentration samples. The applicability was also evaluated by testing 20 maternal plasma samples.@*RESULTS@#The ddPCR method could accurately detect the beta-thalassemia CD41-42 mutation in cell-free DNA derived from maternal plasma. Within the target sequence concentration ratio of 5.00%-0.50%, the relative errors were all < 0.05, the linear regression equation was Y=1.0101-X-0.0071 and R=0.9994. The results of 20 maternal plasma cell-free DNA samples were all consistent with those of the follow-up testing.@*CONCLUSION@#A ddPCR method for detecting parental CD41-42 mutation in cell-free DNA from maternal plasma was developed. The method is simple, rapid, accurate, and can be applied for non-invasive prenatal diagnosis for couples simultaneously carrying the CD41-42 mutation.